alkaline lysis meaning in English
碱裂解（法）
Examples
- An improved alkaline lysis adaptive to extracting plasmid dna of bacillus
提取的改良碱裂解法 - Methods 1 . the mtdnas from fmmu albino and pigment guinea pigs were extracted with alkaline lysis
方法： 1 、用碱变性法提取fmmu白化豚鼠以及花色豚鼠的mtdna 。 - The presence of plasmid in bacillus was isolated by improved alkaline lysis . only one plasmid pbl29 was detected in bacillus iicheniformis29
本文采用改良碱裂解法对30株益生芽孢杆菌进行质粒抽提，仅从一株地衣芽孢杆菌b - Materials and methods in our study , first the e . coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis . the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i . the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis
同预想结果基本吻合，大规模培养后纯化得到的融合蛋白通过sds page显示在52kda处有一条带，而酶切后产物电泳结果显示在26kda处分另有两条蛋白带，其中一条为gst ，另一条为hbrp ，基本符合实验结果； hbrp对tpk活性的抑制呈剂量依赖性。